Rock Imager 2.9: Autoscoring for UV and SONICC images; Image masking to reduce plate fluorescence

April 08, 2013 by Astri Leave a Comment

Rock Imager 2.9 introduces autoscoring for UV and SONICC images as well as image masking, which can improve imaging results by "masking" fluorescence from certain plate types.

Auto Scoring. With Rock Imager 2.9, your time spent reviewing images that show empty wells and drops without crystal growth is coming to an end. Based on proprietary algorithms around image intensity, sharpness, edges, and objects, Rock Imager now categorizes each Ultraviolet, UV-TPEF or SHG image as a positive or  negative hit. Using this feature, you can choose to filter your images for only positive hits or both positive and negative hits in Rock Maker.

The examples below are UV-TPEF images acquired with a SONICC imager. Mousing over the score in Rock Maker displays basic information about the score's origin, e.g. "UV-TPEF Auto Score: Positive Hit".

RI 29 AS UVTPEF-II

You can also filter the Canvas view to show only drops with positive hits from each imager, or drops with a positive hit from any imager. For more information about these capabilities, please see the Rock Maker help file.

Based on initial results, our algorithm yields less than 2 % false negatives and less than 8 % false positives. We hope to continually improve our detection algorithms for better accuracy with your help.

9-16-2015 3-48-25 PM

Note: You must be using Rock Maker 2.7 to view auto scores.

Image Masking. Image Masking can be used to hide fluorescence that appears around the edges of certain plate types containing specific materials. By hiding irrelevant fluorescence, plate masks reduce the probability of a false positive and increase the accuracy of Rock Imager’s auto score capability.

ImagerLink Changes on Rock Imager 2.9 (ImagerLink v 2.7.0.7). To complement the autoscoring feature, some options have been added to ImagerLink.

Posted in: Rock Imager, Rock Imager 2.9, What's New, PC Software Updates

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