Rock Imager 3.0 brings some big improvements for all types of imagers and light paths to make your work easier. Here is a quick look at some of the new developments for Rock Imager 3.0.
FRAP data is retained even when focus level saving is disabled
Individual focus level images and still retain all the associated FRAP information. All associated FRAP images, such as pre-bleach, end-state and charts, will be saved regardless of database management and whether or not visible or UV focus level images are saved.
Validation added to ensure accurate FRAP results
When analyzing a plate with High Throughput FRAP, Rock Imager uses a software algorithm to align the pre-bleach and end-state images. If the algorithm fails to align the images within the specified offset tolerance, no FRAP results are calculated. Instead, an image with an error message is displayed in Rock Maker and a score of “-1” is given, alerting you to the FRAP analysis failure.
Rock Imager 1000 and 182
Move multiple plates to the load port
Managing plates is easier now that Rock Imager enables you to move any number of plates to the load port at once, saving you time. If the load port is currently full, the plates will remain queued and automatically be moved as space becomes available.
Adjust the Field of View (FOV) with continuous zoom imaging
In addition to the multiple objectives available, Rock Imager MFI allows for continuous zoom imaging. This control enables you to tighten the FOV perfectly to the edges of your drops and is achieved by cropping the images acquired by the camera. The FOV for fluorescent images will match the FOV for visible images for comparison purposes.
All Rock Imagers
Optimized plate handling
With optimized plate handling, Rock Imager will no longer acquire drop overview images for drop locations that have previously been defined in Rock Maker. This is especially useful for double imagers, allowing plates to be imaged immediately in SONICC without first having to go to the visible imager to acquire an overview image.
Other ways Rock Imager 3.0 can make life easier:
- The display for resolution and image size have been split up and renamed as “Binning” and “Image Size” to accurately display the size of the image in pixels. For MFI images, the image size will be affected by the zoom control and image cropping on the camera.
- Labels for the temperature sensor were changed from "Mid Back" and "Top R" to “Middle” and “Top”.
Many of the best Rock Imager updates have been suggested by our awesome user community.
Have a great idea on how to make Rock Imager better? Let us know by contacting us at firstname.lastname@example.org!