Multi-Fluorescence Imaging of Protein Crystals

April 24, 2017 by Lance Ramsey Leave a Comment
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Posted in: membrane proteins, Protein Crystallization, protein crystals, protein-protein complexes, imaging

Detection and Imaging of Protein Microcrystals using SONICC

April 06, 2017 by Lance Ramsey Leave a Comment

Authors: Ellen Gualtieri, Eric Zhao, Lance RamseyAbout SONICCSONICC, Second Order Non-linear Imaging of Chiral Crystals, uses a femtosecond pulsed laser to exploit the frequency-doubling effect found in the majority of protein crystals, and...

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Posted in: imaging, Protein Crystallization, lcp, SONICC, membrane proteins, protein crystals

Use of FRAP to Filter Out Non-Crystallizing Conditions

March 24, 2017 by Lance Ramsey Leave a Comment

Authors: Ellen Gualtieri1, Eugene Chun2, Wei Liu2, Lance Ramsey11Formulatrix, Inc. 2Arizona State University

Problem:Finding the optimal conditions to crystallize integral membrane proteins is time consuming. Crystals can take two weeks or more...

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Posted in: imaging, Protein Crystallization, FRAP, lcp, prescreening conditions

Multi-Fluorescent Imaging for Sensitive Protein Crystallization Detection

February 21, 2017 by Lance Ramsey Leave a Comment

Problem:The unambiguous and reliable identification of biological crystals remains a major obstacle in crystallography, particularly in the critical stage of initial screening experiments. Automated imaging at high-resolution in the visible range...

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Posted in: protein-protein complexes, imaging, Protein Crystallization, UV

Imaging of Lipidic Cubic Phase (LCP) Plates

December 23, 2016 by Lance Ramsey Leave a Comment

Those conditions that are determined as optimal for crystallization per FRAP are subsequently used as the basis for a crystallization screen. After an extended period of time, the crystallization plates are imaged in the hopes of finding well-formed...

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Posted in: Protein Crystallization, lipidic cubic phase, lcp, imaging

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