Fluorescence Recovery After Photobleaching (FRAP) Group on LinkedIn

July 31, 2017 by Lance Ramsey Leave a Comment

Formulatrix has started to new LinkedIn group for those interested in or currently using Fluorescence Recovery After Photobleaching (FRAP) for pre-screening protein crystallization conditions.  

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Posted in: Protein Crystallization, FRAP, lcp, membrane proteins

Prescreening Membrane Protein Crystallization Conditions with FRAP

April 28, 2017 by Lance Ramsey Leave a Comment

Authors: Lance Ramsey, Ellen Gualtieri, Eric Zhao

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Posted in: Protein Crystallization, FRAP, lcp, prescreening conditions, membrane proteins, protein crystals

Cold Spring Harbor Asia - Membrane Proteins: Structure & Function

April 10, 2017 by Lance Ramsey Leave a Comment
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Posted in: Protein Crystallization, Rock Maker, NT8, Rock Imager, SONICC, FRAP, lipidic cubic phase, lcp, mfi, membrane proteins

Detection and Imaging of Protein Microcrystals using SONICC

April 06, 2017 by Lance Ramsey Leave a Comment

Authors: Ellen Gualtieri, Eric Zhao, Lance RamseyAbout SONICCSONICC, Second Order Non-linear Imaging of Chiral Crystals, uses a femtosecond pulsed laser to exploit the frequency-doubling effect found in the majority of protein crystals, and...

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Posted in: Protein Crystallization, SONICC, imaging, lcp, membrane proteins, protein crystals

Use of FRAP to Filter Out Non-Crystallizing Conditions

March 24, 2017 by Lance Ramsey Leave a Comment

Authors: Ellen Gualtieri1, Eugene Chun2, Wei Liu2, Lance Ramsey11Formulatrix, Inc. 2Arizona State University

Problem:Finding the optimal conditions to crystallize integral membrane proteins is time consuming. Crystals can take two weeks or more...

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Posted in: Protein Crystallization, FRAP, imaging, lcp, prescreening conditions

Imaging of Lipidic Cubic Phase (LCP) Plates

December 23, 2016 by Lance Ramsey Leave a Comment

Those conditions that are determined as optimal for crystallization per FRAP are subsequently used as the basis for a crystallization screen. After an extended period of time, the crystallization plates are imaged in the hopes of finding well-formed...

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Posted in: Protein Crystallization, imaging, lipidic cubic phase, lcp

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